C7831

Sigma

 

Monoclonal Anti-CD28 antibody produced in mouse

clone CD28.2, purified immunoglobulin, buffered aqueous solution

Synonym:Monoclonal Anti-CD28

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Description

Antigen BackgroundCD28 is expressed on thymocytes, peripheral lineage cell subsets, activated B cells and plasma cells. The antigen is involved in adhesion between T and B lymphocytes through the B7/BB-1 ligand expressed on activated B cells. CD28 co-stimulates T-cells activated via the CD3 T-cell receptor and CD2 pathways and induces their proliferation.
Immunogenhuman CD28 transfected murine T cell hybridoma.
SpecificityRecognizes the CD28 (44 kDa) human cell surface disulfide-linked, homodimeric glycoprotein. The antibody reacts with human CD28-transfected murine T-cell hybridoma, PHA-activated blasts and IL-6 dependent plasmacytoma lines. It cross reacts with rhesus monkey PBL. The antibody blocks CD28/B7 (CD80) mediated cell-cell adhesion. It binds to the CDR domain in a CDR1 dependent manner. It enhances CD3 monoclonal antibody-induced T-cell proliferation. May be used in adhesion and co-stimulation studies.
5th Workshop: code no. CD28.05.
Physical formSolution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Properties

antibody formpurified immunoglobulin
cloneCD28.2 (monoclonal)
formbuffered aqueous solution
mol wtantigen mol wt 44 kDa
species reactivityrhesus monkey, human
application(s)flow cytometry: 5 μL using 1 × 106 cells
 immunocytochemistry: suitable
 immunohistochemistry (frozen sections): suitable
isotypeIgG1
Gene Informationhuman ... CD28(940)
shipped inwet ice
storage temp.2-8°C

Safety

WGK Germany3

References

referenceSchlossman, S.F., et al. Leucocyte Typing V Oxford , (1995), 360
 Nunes, J., et al. Int. Immunol. 5, 311, (1993)